2024

Scientific Reports: Validation of a functional human AD model with four AD therapeutics utilizing patterned ipsc-derived cortical neurons integrated with microelectrode arrays.

• J. Caneus, K. Autar, N. Akanda, M. Grillo, C. J. Long, M. Jackson, S. Lindquist, X. Guo, D. Morgan, and J. J. Hickman
• Published 22 October 2024
• DOI: 10.1038/s41598-024-73869-9

ACS Biomaterials Science & Engineering: Establishment of a Serum-Free Human iPSC-Derived Model of Peripheral Myelination

• A. Patel, M. Williams, K. Hawkins, L. Gallo, M. Grillo, N. Akanda, X. Guo, S. Lambert, and J. J. Hickman
• Published 22 October 2024
• DOI: 10.1021/acsbiomaterials.4c01431

Alzheimer's & Dementia:  A functional aged human iPSC-cortical neuron model recapitulates Alzheimer's disease, senescence, and the response to therapeutics

• L. H. Gallo, N. Akanda, K. Autar, A. Patel, I. Cox, H. A. Powell, M. Grillo, N. Barakat, D. Morgan, X. Guo, and J. J. Hickman
• Published 30 July 2024
• DOI: 10.1002/alz.14044

ACS Biomaterials Science & Engineering:  Mimicking the Tendon Microenvironment to Enhance Skeletal Muscle Adhesion and Longevity in a Functional Microcantilever Platform

• H. Jangir and J. J. Hickman
• Published 18 July 2024
• DOI: 10.1021/acsbiomaterials.3c00235

Lab on a Chip:  Modelling the innate immune system in microphysiological systems

• M. J. Rupar, H. Hanson, S. Rogers, B. Botlick, S. Trimmer, and J. J. Hickman
• Published 3 July 2024. 
• DOI:  10.1039/d3lc00812f

Lab on a Chip:  From animal testing to in vitro systems: advancing standardization in microphysiological systems.

• D.R. Reyes, M. Esch, L. Ewart, R. Nasiri, A. Herland, K. Sung, M. Piergiovanni, C. Lucchesi, J.T. Shoemaker, J. Vukasinovic, H. Nakae, J. Hickman, K. Pant, A. Taylor, N. Heinz, N. Ashammakhi.
• Published 15 February 2024
• DOI: 10.1039/D3LC00994G

Biomedicines: Inhibition of Metalloproteinases Extends Longevity and Function of In Vitro Human iPSC-Derived Skeletal Muscle.

• Barakat, N.; Jangir, H.; Gallo, L.; Grillo, M.; Guo, X.; Hickman, J.
• Published 12 April 2024
• DOI: 10.3390/biomedicines12040856

Hesperos Research Scientists in bold

2023

Advanced Biology: Human IPSC-Derived PreBötC-Like Neurons and Development of an Opiate Overdose and Recovery Model

• Xiufang Guo, Nesar Akanda, Gabriella Fiorino, Siddharth Nimbalkar, Christopher J. Long, Alisha Colón, Aakash Patel, Patrick J. Tighe, James J. Hickman

• Published 7 September 2023

• DOI: 10.1002/adbi.202300276

Scientific Reports: Development of a Human Malaria-on-a-Chip Disease Model for Drug Efficacy and Off-Target Toxicity Evaluation

• M.J. Rupar, T. Sasserath, E. Smith, B. Comiter, N. Sriram, C.J. Long, C.W. McAleer, J.J. Hickman
• Published 28 June 2023
• DOI: 10.1038/s41598-023-35694-4

ACS Biomaterials Science & Engineering: Mimicking tendon microenvironment to enhance skeletal muscle adhesion and longevity in a functional microcantilever platform

• H. Jangir, J.J. Hickman
• Published 18 July 2023
• DOI: 10.1021/acsbiomaterials.3c00235

International Journal of Molecular Sciences: The Effect of Skeletal Muscle-Specific Creatine Treatment on ALS NMJ Integrity and Function.

• Badu-Mensah, A., Guo, X., Mendez, R., Parsaud, H., & Hickman, J. J
• Published 4 August 2023
• DOI: 10.3390/ijms241713519

Frontiers in Cell and Developmental Biology: Development of a functional human induced pluripotent stem cell-derived nociceptor MEA system as a pain model for analgesic drug testing.

• Nimbalkar, S., Guo, X., Colon, A., Jackson, M., Akanda, N., Patel, A., Grillo, M., & Hickman, J. J. 
• Published 1 March 2023
• DOI: 10.3389/fcell.2023.1011145

Hesperos Research Scientists in bold

Biomaterials: ALS mutations in both human skeletal muscle and motoneurons differentially affects neuromuscular junction integrity and function

• A. Badu-Mensah, X. Guo, S. Nimbalkar, Y. Cai, J.J. Hickman
• Published 19 August 2022
• DOI: 10.1016/j.biomaterials.2022.121752

Advanced Therapeutics: An induced pluripotent stem cell-derived NMJ platform for study of the NGLY1-congenital disorder of deglycosylation

• T. Sasserath, A.L. Robertson, R. Mendez, T.T. Hays, E. Smith, H. Cooper, N. Akanda, J.W. Rumsey, X. Guo, M. Might, S. Rodems, K. Baumgaertel, M. Pradhan, W. Zheng, A. Farkhondeh and J.J. Hickman
• Published 15 July 2022
• DOI: 10.1002/adtp.202200009

Nature Reviews Methods Primers: A guide to the organ-on-a-chip.

• Leung, C. M., de Haan, P., Ronaldson-Bouchard, K., Kim, G-A., Ko, J., Rho, H. S., Chen, Z., Habibovic, P., Jeon, N. L., Takayama, S., Shuler, M. L., Vunjak-Novakovic, G., Frey, O., Verpoorte, E., & Toh, Y-C.
• Published 12 May 2022
• DOI: 10.1038/s43586-022-00118-6

Advanced Therapeutics: Classical Complement Pathway Inhibition in a ‘Human-on-a-Chip’ Model of Autoimmune Demyelinating Neuropathies

• J.W. Rumsey, C. Lorance, M. Jackson, T. Sasserath, C.W. McAleer, C.J. Long, A. Goswami, M.A. Russo, S.M. Raja, K.L. Gable, D. Emmett, L.D. Hobson-Webb, M. Chopra, J.F. Howard, Jr., J.T. Guptill, M.J. Storek, M. Alonso-Alonso, N. Atassi, S. Panicker, G. Parry, T. Hammond, J.J. Hickman
• Published 5 April 2022
• DOI: 10.1002/adtp.202200030

Stem Cell Reports: A functional hiPSC-cortical neuron differentiation and maturation model and its application to neurological disorders

• K. Autar, X. Guo, J.W. Rumsey, C. Long, N. Akanda, M. Jackson, N.S. Narasimhan, J. Caneus, D. Morgan, J.J. Hickman
• Published 11 Jan 2022
• DOI: 10.1016/j.stemcr.2021.11.009

Hesperos Research Scientists in bold

Frontiers in Cell and Developmental Biology: A Functional Human-on-a-Chip Autoimmune Disease Model of Myasthenia Gravis for Development of Therapeutics

• V.M. Smith, H. Nyguen, J.W. Rumsey, C.J. Long, M.L. Shuler and J.J. Hickman
• Published 21 November 2021
• DOI: 10.3389/fcell.2021.745897

The Neuroscience Chronicles: ALS Skeletal Muscle: Victim or Culprit

• A. Badu-Mensah, X. Guo and J.J. Hickman
• Published 28 June 2021
• DOI: doi.org/10.46439/Neuroscience.2.012

Nature Scientific Reports: Validation of an adipose‑liver human‑on‑a‑chip model of NAFLD for preclinical therapeutic efficacy evaluation

• V.L. Slaughter, J.W. Rumsey, R. Boone, D. Malik, Y. Cai, N.N. Sriram, C.J. Long, C.W. McAleer, S. Lambert, M.L. Shuler and J.J. Hickman
• Published 23 June 2021
• DOI: 10.1038/s41598-021-92264-2

Clinical and Translational Science: 3D Cell Culture Models: Drug Pharmacokinetics, Safety Assessment, and Regulatory Consideration

• H. Wang, P.C. Brown, E.C.Y. Chow, L. Ewart, S.S. Ferguson, S. Fitzpatrick, B.S. Freedman, G. Guo, W. Hedrich, S. Heyward, J. Hickman, N. Isoherranen, A.P. Li, Q. Liu, S.M. Mumenthaler, J. Polli, W.R. Proctor, A.P. Ribeiro, J.-Y. Wang, and R.L. Wange and S.-M. Huang
• Published 13 May 2021
• DOI: 10.1111/cts.13066

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ALTEX: New approach methodologies (NAMs) for human-relevant biokinetics predictions

• Ans Punt, Hans Bouwmeester, Bas J. Blaauboer, Sandra Coecke, Betty Hakkert, Delilah F. G. Hendriks, Paul Jennings, Nynke I. Kramer, Sibylle Neuhoff, Rosalinde Masereeuw, Alicia Paini, Ad A. C. M. Peijnenburg, Martijn Rooseboom, Michael L. Shuler, Ian Sorrell, Bart Spee, Marije Strikwold, Andries D. van der Meer, Meike van der Zande, Mathieu Vinken, Huan Yang, Peter M. J. Bos, Minne B. Heringa

• Published 20 October 2020

• DOI: 10.14573/altex.2003242

Scientific Reports: Functional skeletal muscle model derived from SOD1-mutant ALS patient iPSCs recapitulates hallmarks of disease progression

• Agnes Badu-Mensah, Xiufang Guo, Christopher McAleer, John W. Rumsey & James J. Hickman 

• Published 31 August 2020

• DOI: 10.1038/s41598-020-70510-3

Advanced Therapeutics: A Human-Based Functional NMJ System for Personalized ALS Modeling and Drug Testing

• Xiufang Guo, Virginia Smith, Max Jackson, My Tran, Michael Thomas, Aakash Patel, Eric Lorusso, Siddarth Nimbalkar, Yunqing Cai, Christopher McAleer, Ying Wang, Christopher Long, James Hickman

• Published 11 August 2020

• DOI: 10.1002/adtp.202000133

Advanced Science: Differential Monocyte Actuation in a Three-Organ Functional Innate Immune System on a Chip

• Trevor Sasserath, John Rumsey, Christopher McAleer, Lee Richard Bridges, Christopher Long, Daniel Elbrecht, Franz Schuler, Adrian Roth, Christina Bertinetti-LaPatki, Michael Shuler, James Hickman

• In collaboration with Roche Pharmaceuticals

• Published 02 June 2020

• DOI: 10.1002/advs.202000323

Alzheimers & Dementia: A human induced pluripotent stem cell-derived cortical neuron human-on-a chip system to study Aβ42 and tau-induced pathophysiological effects on long-term potentiation

• Julbert Caneus, Nesar Akanda, John W. Rumsey, Xiufang Guo, Max Jackson, Christopher J. Long, Frank Sommerhage, Sanya Georgieva, Nicholas M. Kanaan, David Morgan, James J. Hickman

• Published 27 May 2020

• DOI: 10.1002/trc2.12029

Lab on a Chip: Microphysiological heart-liver body-on-a-chip system with skin mimic for evaluating topical drug delivery

• Camilly P. Pires de Mello, Carlos Carmona-Moran, Christopher W. McAleer, Julian Perez, Elizabeth A. Coln, Christopher J. Long, Carlota Oleaga, Anne Riu, Reine Note, Silvia Teissier, Jessica Langer, James J. Hickman

• Published 17 Jan 2020

• DOI: 10.1039/C9LC00861F

Hesperos Research Scientists in bold

APL Bioengineering: Microphysiological systems

•  J. J. Hickman, D Huh, R. D. Kamm

• Published 29 October 2019 

• DOI: 10.1063/1.5130170

Biotechnology and Bioengineering: Multi-organ microfluidic platform with breathable lung chamber for inhalation or intravenous drug screening and development.

• Shuler M.L., Miller PM, Chen CY, Wang YI, Gao E 

• Published 14 October 2019 

• DOI: 10.1002/bit.27188

APL Bioengineering: A human in vitro platform for the evaluation of pharmacology strategies in cardiac ischemia

• C. Oleaga, G. Jalilvand, G. Legters, C. Martin, G. Ekman, C. W. McAleer, C. J. Long, J. J. Hickman

• Published 13 August 2019

• DOI: 10.1063/1.5089237

ACS Chemical Neuroscience in press: Physiological Aβ Concentrations Produce a More Biomimetic Representation of the Alzheimer’s Disease Phenotype in iPSC Derived Human Neurons.

• B. Berry, A.S.T. Smith, C. J. Long, C. Martin, and J. J. Hickman

• Published 18 July 2019 

• DOI: 10.1021/acschemneuro.8b00067

Lab on a Chip: A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells.

• Chen H.J., P. Miller, M.L. Shuler 

• Published 10 July 2019

• DOI: 10.1039/c8lc00111a

Nature Scientific Reports: On the Potential of In Vitro Organ-Chip Models to Define Temporal Phamacokinetic-Pharmacodynamic Relationships

• C. W. McAleer, A. Pointon, C. J. Long, R> Brighton, B. Wilkin, L. R. Bridges, N. Sriram, K. Fabre, R. McDougall, V. Muse, J. Mettetal, A. Srivastava, D. Williams, M. Schnepper, J. Roles, M. L. Shuler, J. J. Hickman

• Published 3 July 2019

• DOI: 10.1038/s41598-019-45656-4

Science Translational Medicine: Multi-Organ System for the Evaluation of Efficacy and Off-Target Toxicity of Anticancer Therapeutics

• C. W. McAleer, C. J. Long, D. Elbrecht, T. Sasserath, L. R. Bridges, J. Rumsey, C. Martin, M. Schnepper, Y. Wang, F. Schuler, A. Roth, C. Funk, M. L. Shuler, J. J. Hickman

• Published 19 June 2019 

• DOI:10.1126/scitranslmed.aav1386

• Press Release

Hesperos Research Scientists in bold

Advanced Functional Materials: Long-Term Electrical and Mechanical Function Monitoring of a Human-on-a-Chip System

• C. Oleaga, A. Lavado, A. Riu, S. Rothemund, C. A. Carmona-Moran, K. Persaud, A. Yurko, J. Lear, N. Sriram, C. J. Long, F. Sommerhage, L. R. Bridges, Y. Cai, C. Martin, M. T. Schnepper, A. Goswami, R. Note, J. Langer, S. Teissier, J. Cotovio, and J. J. Hickman
• Published 14 December 2018
• DOI: 10.1002/adfm.201805792

AIChE Journal: Application of Chemical Reaction Engineering Principles to “Body-on-a-Chip” Systems

• Sung, Jong Hwan, Y. Wang, J. Min Lee, and M. Shuler
• Published 12 October 2018
• PMID: 31402795

Lab Chip: UniChip enables long-term recirculating unidirectional perfusion with gravity-driven flow for microphysiological systems

• Wang, Y.I. and M.L. Shuler
• Published 20 July 2018
• DOI: 10.1039/C8LC00394G

Lab Chip: A pumpless body-on-a-chip model using a primary culture of human intestinal cells and a 3D culture of liver cells

• Chen H.J., P. Miller , M.L. Shuler
• Published 4 June 2018
• DOI: 10.1039/c8lc00111a

ACS Chem Neurosci: Physiological Aβ Concentrations Produce a More Biomimetic Representation of the Alzheimer’s Disease Phenotype in iPSC Derived Human Neurons

• B. Berry, A.S.T. Smith, C. J. Long, C. Martin and J.J. Hickman
• Published 10 May 2018
• DOI: 10.1021/acschemneuro.8b00067

Biomaterials: Stem Cell Derived Phenotypic Human Neuromuscular Junction Model for Dose Response Evaluation of Therapeutics

• N. Santhanam, L. Kumanchik, X. Guo, F. Sommerhage, Y. Cai, M. Jackson, C. Martin, G. Saad, C.W. McAleer, Y. Wang, A. Lavado, C.J. Long, J.J. Hickman
• Published 27 February 2018
• DOI: 10.1016/j.biomaterials.2018.02.047

ALTEX: Fetal Bovine Serum (FBS) Past – Present – Future

• J. van der Valk, K. Bieback, C. Buta, B. Cochrane, W.G. Dirks, J. Fu, J.J. Hickman, C. Hohensee, R. Kolar, M. Liebsch, F. Pistollato, M. Schulz, D. Thieme, T. Weber, J. Wiest, S. Winkler and G. Gstraunthaler
• Published 17 January 2018
• DOI: 10.14573/altex.1705101

Hesperos Research Scientists in bold

International Journal of Pharmacy and Pharmaceuticals: Evaluation of Holistic Treatment for ALS Reveals Possible Mechanism and Therapeutic Potential

• A. Lavado, X. Guo, N. Akanda, C. Martin, Y. Cai, D. Elbrecht, M. Tran, J.-P. Bryant, A. Colon, C.J. Long, S. Lambert, D. Morgan and J.J Hickman
• Published 30 December 2017
• PMID: 30637316

Nature Scientific Reports: Functional Analysis of Human Intrafusal Fiber Innervation by Human γ-Motoneurons

• A. Colon, X. Guo, N. Akanda, Y. Cai and J.J. Hickman
• Published 8 December 2017
• DOI: 10.1038/s41598-017-17382-2

Advanced Healthcare Materials: Multi-Organ Microphysiological Systems for Drug Development: Strategies, Advances and Challenges

• Y.I. Wang, C. Carmona, J.J. Hickman and M.L. Shuler
• Published 4 December 2017
• DOI: 10.1002/adhm.201701000

ACS Biomaterials Science & Engineering: Comparison of NMDA and AMPA Channel Expression and Function between Embryonic and Adult Neurons Utilizing Microelectrode Array Systems

• D. Edwards, F. Sommerhage, B. Berry, H. Nummer, B. Clymer, M. Stancescu, J.J. Hickman
• Published 13 November 2017
• DOI: 10.1021/acsbiomaterials.7b00596

Annual Review of Pharmacology and Toxicology: Application of Microphysiological Systems to Enhance Safety Assessment in Drug Discovery

• L. Ewart, E.-M. Dehne, K. Fabre, Susan Gibbs, J. Hickman, E. Hornberg, M. Ingelman-Sundberg, K.-J. Jang, D.R. Jones, V.M. Lauschke, U. Marx, Jerome T. Mettetal, A. Pointon, D. Williams, W.-H. Zimmermann, and P. Newham
• Published 13 October 2017
• DOI: 10.1146/annurev-pharmtox-010617-052722

Experimental Biology and Medicine Online: Self-contained, Low-cost Body-on-a-Chip Systems for Drug Development

• Y. Wang, C. Oleaga, C. Long, M.B. Esch, C. McAleer, P.G. Miller; J.J. Hickman and M.L. Shuler
• Published 17 February 2017
• DOI: 10.1177/1535370217694101

Biomaterials: Tissue Engineering the Mechanosensory Circuit of the Stretch Reflex Arc with Human Stem Cells: Sensory Neuron Innervation of Intrafusal Muscle Fibers

• X. Guo, A. Colon,  N. Akanda,  S. Spradling, M. Stancescu, C. Martin, J.J. Hickman
• Published 24 January 2017
• DOI: 10.1016/j.biomaterials.2017.01.005

Hesperos Research Scientists in bold

Altern Lab Anim.: Body-on-a-chip systems for animal-free toxicity testing

• Mahler G.J., Esch M.B., Stokol T., J.J. Hickman, M.L. Shuler
• Published 1 October 2016
• DOI: 10.1177/026119291604400508

Biotechnol Bioeng.: Microfluidic blood-brain barrier model provides in vivo-like barrier properties for drug permeability screening

• Wang Y.I., Abaci H.E., M.L. Shuler
• Published 21 July 2016
• DOI: 10.1002/bit.26045

Nat Biotechnol.: A recellularized human colon model identifies cancer driver genes

• Chen, H.J., Z. Wei, J. Sun, A. Bhattacharya, D.J. Savage, R. Serda, Y. Mackeyer, S.A. Curley, P. Bu, L. Wang, S. Chen, L. Cohen-Gould, N.A. Jenkins, and M.L. Shuler
• Published 11 July 2016
• DOI: 10.1038/nbt.3586

Lab on a Chip: Modular, pumpless body-on-a-chip platform for the co-culture of GI tract epithelium and primary liver tissue

• Esch, M.B., H. Ueno, D.R. Applegate, and M.L. Shuler
• Published 22 June 2016
• DOI: 10.1039/C6LC00461J

Biotechnol Bioeng.: Design and demonstration of a pumpless 14 compartment microphysiological system

• Miller, P.G., and M.L. Shuler
• Published 12 April 2016
• DOI: 10.1002/bit.25989

E Bio Medicine: Modeling barrier tissues in vitro: methods, achievements, and challenges

• Sakolish, C.M., M.B. Esch, J.J. Hickman, M.L. Shuler, and G.J. Mahler
• Published 13 February 2016
• PMID: 27077109

Scientific Reports: Multi-Organ toxicity demonstration in a functional human in vitro system composed of four organs

• C. Oleaga, C. Bernabini, A.S.T. Smith, B. Srinivasan, M. Jackson, W. McLamb, V. Platt, R. Bridges, Y. Cai, N. Santhanam, B. Berry, S. Najjar, N. Akanda, X. Guo, C. Martin, G. Ekman, M.B. Esch, J. Langer, G. Ouedraogo, J. Cotovio, L. Breton, M.L. Shuler and J.J. Hickman
• Published 3 February 2016
• 10.1038/srep20030

Hesperos Research Scientists in bold

Nat Biotechnol.: Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting

• Chen, H.J., Sun, J., Hou, H., Huang, Z., Arcilla, M., Rakhilin, N., Joe, D., Choi, J., Gadamsetty, P., Milsom, J., Nadakumar G., Longman, R., Zhou, X., Edwards, R., Chen, J., Chen, K., Bu, P., Waqng, L., Xu, Y., Munroe, R., Abratte, C., Miller, A., Shuler, M.L., Edelmann, W., Gumus, Z.H., Nishimura, N., Shen, X., and S.M. Lipkin
• Published 25 May 2015
• DOI: 10.1038/nbt.3239 

Lab Chip: Multi-cellular 3D human primary liver cell culture elevate metabolic activity under fluidic flow

• Esch, M.B., Prot, J, Wang, Y.I., Miller, P., Llamas-Vidales, J.R., Naughton, B.A., Applegate, D.R., and M.L. Shuler
• Published 21 May 2015
• DOI: 10.1039/c5lc00237k

Biomaterials: A phenotypic in vitro model for the main determinants of human whole heart function

• M. Stancescu, P. Molnar, C. McAleer, W. McLamb, C. Long, C. Oleaga, J.-M. Prot and J.J. Hickman
• Published 14 May 2015
• DOI: 10.1016/j.biomaterials.2015.04.035

J Lab Automation: TEER measurement techniques for in vitro barrier model systems

• Srinivasan, B., Kolli, A.R., Esch, M.B., Abaci, H.E., Shuler, M.L., and J.J. Hickman
• Published April 2015
• DOI: 10.1177/2211068214561025

Integr Biol: Human-on-a-chip design strategies and principles for physiologically based pharmacokinetics/pharmacodynamics modeling

• Abaci, H.E., and M.L. Shuler
• Published 5 March 2015
• DOI: 10.1039/c4ib00292j

Lab Chip: Pumpless microfluidic platform for drug testing on human skin equivalents

• Abaci, H.E., Gledhill, K., Guo, Z., Christiano, A.M., and M.L. Shuler
• Published 7 February 2015
• DOI: 10.1039/c4lc00999a

Biotechnology Progress: Functional Myotube Formation from Adult Rat Satellite Cells in a Defined Serum-Free System

• C. McAleer, J.W. Rumsey, M. Stancescu and J.J. Hickman
• Published 16 February 2015
• DOI: 10.1002/btpr.2063

Hesperos Research Scientists in bold

• C.W. McAleer, A.S.T. Smith, S. Najjar, K. Pirozzi, C.J. Long and J.J. Hickman, “Mechanistic Investigation of Adult Myotube Response to Exercise and Drug Treatment in Vitro Using a Multiplexed Functional Assay System,” J Appl Physiol 117(11):1398-1405 (2014).

• Esch, M.B., Smith, A., Prot, J.M., Oleaga, C., Hickman, J., and Shuler, M.L. 2014. How Multi-Organ Microdevices Can Help Foster Drug Development. Advanced Drug Delivery Reviews, 69-70 (2014) 158-169.

• Esch, M.B., Mahler, G.J., Stokol, T., and M.L. Shuler. 2014. Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury. Lab Chip, 14(16): 3081-92. DOI: 10.1039/c4lc00371c.

• Shuler, M.L. and J.J. Hickman. 2014. Toward in virtro models of brain structure and function. PNAS. 111(38): 13682-13683.

• Sung, J.H., Srinivasan, B., Esch, M.B., McLamb, W.T., Bernabini, C., Shuler, M.L., and J.J. Hickman. 2014. Using physiologically-based pharmacokinetic-guided “body-on-a-chip” systems to predict mammalian response to drug and chemical exposure. Experimental Biology and Medicine. 239:1225-1239.

• Bocioaga, D., I.A. El Gheriany, L.W. Lion, W.C. Ghiorse, M.L. Shuler and A.G. Hay. 2014. Development of a genetic system for a model manganese-oxidizing proteobacterium, Leptothrix discophora SS1. Microbiology. Bo: 2396-2405.

• X. Guo, K. Greene, N. Akanda, A.S.T. Smith, M. Stancescu, S. Lambert, H. Vandenburgh, and J.J. Hickman, “In vitro Differentiation of Functional Human Skeletal Myotubes in a Defined System,” Biomaterials Science 2:131-138 (2014).

• A.S.T. Smith, C.J. Long, B.J. Berry, C. McAleer, M. Stancescu, P. Molnar, P.G. Miller, M.B. Esch, J.-M. Prot, J.J. Hickman and M.L. Shuler, “Microphysiological systems and low cost microfluidic platform with analytics,” Stem Cell Research and Therapy in press (2014).

• M. B. Esch, A.S.T. Smith,J.-M. Prot, C. Oleaga, J.J. Hickman and M.L. Shuler “How Multi-Organ Microdevices Can Help Foster Drug Development” ADDR in press (2014).

• J.H. Sung, M.B. Esch, W.T. McLamb, C. Bernabini, B. Srinivasan, M.L. Shuler and J.J. Hickman, “Using PBPK guided “Body-on-a-Chip” Systems to Predict Mammalian Response to Drug and Chemical Exposure,” accepted to Experimental Biology and Medicine (2014).

• A.S.T. Smith, C.J. Long, C. McAleer, N. Bobbitt, B. Srinivasan and J.J. Hickman, “Utilization of microscale silicon cantilevers to assess cellular contractile function in vitro,” accepted to JoVE (2014).

• M. Ramalingam, P, Molnar and J.J. Hickman, “Surface Patterning of Biomaterials to Control Cell Behavior,” Encyclopedia of Nanoscience and Nanotechnology(ENN) American Scientific Publishers, Valencia, California, in press.

• Smith, A.S., Long, C.J., Berry, B.J., Mcaleer, C., Molnar, P., Miller, P.G., Esch, M.B., Prot, J.M., Hickman, J.J. and Shuler, M.L. 2013. Microphysiological systems and low-cost microfluidic platform with analytics. Stem Cell Res Ther. DOI: 10.1186/scrt370. PMID: 24565109. Epub 2013 Dec 20.

• Angus, et al. 2013. A physical sciences network characterization of non-tumorigenic and metastatic cells, Sci. Rep. 3:14493 DOI:10.1038/srep01449.

• S.A. Khan, V. Thakore, A. Behal, L. Bölöni and J.J. Hickman, “Comparative analysis of system identification techniques for nonlinear modeling of the neuron-microelectrode junction,” Journal of Computational and Theoretical Nanoscience, 10(3):573-580 (2013).

• A. Natarajan, T. DeMarse, P. Molnar, J.J. Hickman, “Engineered In Vitro Feed-Forward Networks,” Journal of Biotechnology and Biomaterials 3(1):153 (2013).

• X. Guo, S. Spradling, M. Stancescu, S. Lambert, J.J. Hickman, “Derivation of sensory neurons and neural crest stem cells from human neural progenitor hNP1,” Biomaterials 34:4418-4427 (2013).

• J.H. Sung, M.B. Esch, J.-M. Prot, C.J. Long, A. Smith, J.J. Hickman and M.L. Shuler, “Microfabricated mammalian organ systems and their integration into models of whole animals and humans,” Lab on a Chip 13:1201-1212 (2013).

• C. Finch, T. Clarke, and J.J. Hickman, “A Continuum Hard-Sphere Model of Protein Adsorption,” Journal of Computational Physics, 244:212-222 (2013).

• J.W. Rumsey, C. McAleer, M. Das, A. Bhalkikar, K. Wilson, M. Stancescu, S. Lambert, J.J. Hickman, “Myelination and node of Ranvier formation on sensory neurons in a defined in vitro system,” In Vitro Cell Dev Biol Anim, 49:608-618 (2013).

• K.L. Pirozzi C.J. Long, C.W. McAleer, A.S.T. Smith and J.J. Hickman, “Correlation of embryonic skeletal muscle myotube physical characteristics with contractile force generation on an Atomic Force Microscopy-based bio- Microelectromechanical systems device,” Applied Physics Letters, 103:083108 (2013).

• D. Edwards, M. Stancescu, P. Molnar and J.J. Hickman, “Two cell networks of oriented adult hippocampal neurons on self-assembled monolayers for use in the study of neuronal communication in a defined system,” ACS Chemical Neuroscience 4:1174-1182 (2013) DOI: 10.1021/cn300206k

• A.S.T. Smith, C.J. Long, Kristen Pirozzi and J.J. Hickman, “Development of a functional system for high-content screening of neuromuscular junctions,” Technology, 1(1):37-48 (2013).

• Krasnoff, S.B., U Englich, P.G. Miller, M.L. Shuler, R.P. Glahn, B.G.G. Donzelli, and D.M. Gibson. 2012. Metacridamides A and B, Macrocycles from Conidia of the Entomopathogenic Fungus, Metarhizium acridum. J. Nat Prod. 75: 175-180 DOI: 10.1021/n p 20070441.

• Kim, B.J., T. Zhao, L. Young, P. Zhou, M.L. Shuler. 2012. Batch, fed-batch, and microcarrier cultures with CHO cell lines in a pressure-cycle driven miniaturized bioreactor. Biotechnol and Bioeng. 109:137-45. DOI: 10.1002/bit.23289.

• Mahler, G.J., M.B. Esch, E. Tako, T.L. Southand, S.D. Archer, R.P. Glahn, and M.L. Shuler. 2012. Oral exposure to polystyrene nanoparticles effects iron absorption. Nat Nanotechnol. 7: 264-271 DOI: 10. 1038INNANO.2012.3

• Atlas, J. and M.L. Shuler. 2012. Modeling of a minimal cell. Methods of Mol. Biol. 881: 573-610. DOI: 10.1007/978-1-61779-827-6_20.

• Sung, J.H. and M.L. Shuler, 2012 . Microtechnology for mimicking in vivo tissue environment. Ann. Biomed. Engr. 40: 1289-1300, DOI: 10.1007/s104339-011-0491-2.

• G. Shayan, N. Felix, Y. Cho, M. Chatzichristidi, M.L. Shuler, C.K. Ober, and K.H. Lee. 2012. Synthesis and Characterization of High-Throughout Nanofabricated Poly(4-Hydroxy Styrene) Membranes for In Vitro Models of Barrier Tissue. Tissue Engineering 18:9 667-676 DOI: 10.1089/ten.tec.2011.0598

• Kim, B.J., Diao, J., and M.L. Shuler, 2012. Mini-scale Bioprocessing Systems for Highly Parallel Animal Cell Cultures. DOI:10.1002/btpr.1554. Biotechnol Prog 28: 595-607.

• Shuler, M.L. and J.H. Sung. 2012. Introduction to the special issue on micro- and nanofabrication techniques. Ann Biomed Engr. 40: 1209-1210. DOI: 10.1007/s10439-012-0589-1

• M.L. Shuler. 2012. Modeling Life. Ann Biomed Engr. 40: 1399-407. DOI: 10.1007/s10439-012-0567-7.

• Esch, M.B., Sung, J.H., Yang, J. Yu, J., March, J.C., Shuler, M.L. 2012. On Chip Porous Polymer Membranes for Integration of Gastrointestinal Tract Epithelium with Microfluidic 'Body-on-a-Chip' Devices. Biomedical Microdevices. 14:895-906. DOI: 10.1007/s10544-012-9669-0.

• K.A. Wilson, C. A. Finch, P. Anderson, F. Vollmer, and J.J. Hickman, “Whispering Gallery Mode Biosensor Quantification of Fibronectin Adsorption Kinetics onto Alkylsilane Monolayers and Interpretation of Resultant Cellular Response” Biomaterials 33:225-236 (2012).

• H. Davis, X. Guo, S. Lambert, M. Stancescu and J.J. Hickman, “Small Molecule Induction of Human Umbilical Stem Cells into MBP-Positive Oligodendrocytes in a Defined Three-Dimensional Environment,” ACS Chemical Neuroscience 3:31-39 (2012).

• X. Guo, J.E. Ayala1, M. Gonzalez, M. Stancescu, S. Lambert, J.J. Hickman, “Tissue engineering the monosynaptic circuit of the stretch reflex arc with co-culture of embryonic motoneurons and proprioceptive sensory neurons,” Biomaterials 33(23):5723-5731 (2012).

• C. Long, C. Finch, M. Esch, W. Anderson, M. Shuler and J.J. Hickman, “Design Optimization of Liquid-Phase Flow Patterns for Microfabricated Lung On A Chip,” Annals of Biomedical Engineering 40(6):1255-1267 (2012).

• H. Davis, M. Gonzalez, N. Bhargava, M. Stancescu, J.J. Hickman, and S. Lambert, “Rat Cortical Oligodendrocyte–Embryonic Motoneuron Co-culture: An in vitro Axon-Oligodendrocyte Interaction Model,” Journal of Biomaterials & Tissue Engineering 2(3):206-214 (2012).

• V. Thakore, P. Molnar and J.J. Hickman, “An Optimization Based Study of Equivalent Circuit Models for Representing Recordings at the Neuron-Electrode Interface,” IEEE Transactions on Biomedical Engineering 59(8):2338-2347 (2012).

• C. Wang, J. Reed, L. Ma, Y. Qiao, Y. Luo, S. Zou, J.J. Hickman and M. Su, “Biomimic Light Trapping Silicon Nanowire Arrays for Laser Desorption/Ionization of peptides,” Journal of Physical Chemistry C 116(29):15415-15420 (2012).

• C.N. Baker, D.G. Taylor, K. Osuala, A. Natarajan, P. Molnar, J.J. Hickman, S. Alam, B. Moscato, D. Weinshenker, and S.N. Ebert, “Adrenergic deficiency leads to impaired electrical conduction and increased arrhythmic potential in the embryonic mouse heart,” Biochemical and Biophysical Research Communications 423(3):536-541 (2012).

• X. Guo, J.J. Hickman. “Treatment of ALS utilizing a stem cell strategy” in Motor Neuron Diseases: Causes, Classification and Treatments Eds. B.J. Turner, J.B. Atkin, Nova Science Publishers, Hauppauge, New York. pp.115-136 (2012).

• Shayan, G., Y.S. Choi, E.V. Shusta, M.L. Shuler and K.H. Lee. 2011. Murine in vitro model of the blood brain barrier for evaluating drug transport. Eur. J. Pharm. Sci. 42 148-155.

• Shayan, G., M.L. Shuler, and K.H. Lee. 2011. The effects of astrocytes on the induction of barrier properties in aortic endothelial cells. Biotechnol. Prog. 27:1137-1145.

• Frampton, J.P., M.R. Hynd, M.L. Shuler, W. Shain, 2011. Fabrication and optimization of alginate hydrogel constructs for use in 3D neural cell culture. Biomed. Mat. 6 015002 (18pp).

• Sung, J.S., J. Yu, D. Luo, M.L. Shuler, and J.C. March. 2011. Microscale 3-D hydrogel scaffold for biomimetic gastrointestingal (GI) tract model. Lab Chip. 11, 389-392.

• Esch, M.B., D.J. Post, M.L. Shuler and T. Dec. 2011. Characterization of in vitro endothelial linings grown within microfluidic channels. Tissue Eng. A:17:23-24.

• Seker, E., J.H. Sung, M.L. Shuler, and M.L. Yarmush. 2011. Solving medical problems with BioMEMS. IEEE Pulse. (Nov/Dec) 51-59.

• Esch, M.B., T.L. King and M.L. Shuler. 2011. The role of body-on-a-chip devices in drug and toxicity studies. Ann. Rev. Biomed. Engr. 13:55-72.

• Torres, A., C. Zhu, S. Panullo, and M.L. Shuler. 2011. Paclitaxel delivery to brain tumors from hydrogels: a computationa study. Biotechnol. Prog. 27:1478-1487.

• El Gheriany, I.A., D. Bocjoaga, A.G. Hay, W.C. Ghiorse, M.L. Shuler, and L.W. Lion. 2011. An uncertain role for Cu(II) stimulating Mn(II) oxidation by Leptothrix discophora SS-1. Arch Microbiol. 193: 89-93. DOI: 10.1007/s00203-010-0645-x.

• Xu, H., J. Wu, C. Chu and M.L. Shuler. 2011. Development of disposable PDMS micro cell culture analog devices with photopolymerizable hydrogel encapsulating living cells. Biomed Microdevices. 14: 409-418. DOI: 10.1007/s10544-011-9617-4.

• K. Wilson, M. Stancescu, M. Das, J.W. Rumsey and J.J. Hickman, “Direct Patterning of Coplanar Peg Alkylsilane Monolayers by Deep –Ultraviolet Photolithograpy as a General Method for Long-Term Cell Patterning and Culture,” JVST B 29(2):021020 (2011).

• A. Natarajan, M. Stancescu, V. Dhir, C. Armstrong, F. Sommerhage, J.J. Hickman and P. Molnar, “Patterned Cardiomyocytes on Microelectrode Arrays as a Device for Studying Cardiac Electrophysiology and Drug Effects,” Biomaterials 32(18):4267-4274 (2011).

• X. Guo, M. Gonzalez, M. Stancescu, H. Vandenburgh, J.J. Hickman, “Neuromuscular Junction Formation between Human Stem cell-derived Motoneurons and Human Skeletal Muscle in a Defined System,” Biomaterials 32:9602-9611 (2011).

• Foley, P. and M.L. Shuler. 2010. Considerations for the design and construction of a synthetic platform cell for biotechnological applications. Biotechnol. Bioeng. 105: 26-36.

• Sung, J. H., M.L. Shuler. 2010. In vitro microscale systems for systematic drug toxicity study. Bioprocess Biosyst. Eng. 33: 5-19.

• Sung, J. H., C. Kam, M.L. Shuler. 2010. A microfluidic device for pharmacokinetic-pharmacodynamic (PK-PD) model on a chip. Lab Chip. 10: 446-455.

• Kim, B.J., D.J Schneider, S.W. Cartinhour, and M.L. Shuler. 2010. Complex responses to culture conditions in Pseudomonas syringae pv. tomato DC3000 continuous cultures: the role of iron in cell growth and virulence factor induction. Biotechnol. Bioeng.105: 95-964.

• Choi, J., J.H. Sung, M.L. Shuler, and D. Kim. 2010. Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays. Opt. Lett. 35 (9): 1374-1376.

• Esch, M.B. and M.L. Shuler. 2010. Body-on-a chip: Using microfluidic systems to predict human responses to drugs. Pure Appl. Chem. 82: 1635-1645.

• Sung, J.H., M.B. Esch and M.L. Shuler. 2010. Integration of in silico and in vitro platforms for pharmacokinetic–modeling, Expert Opin. Drug Metab. Toxicol. 6(7): 1-19.

• Esch, M.G., J.H. Sung, and M.L. Shuler. 2010. Challenges, promises and future directions of µCCAs. J. Biotechnol., 148: 64-69.

• Foley, P.L., D.B. Wilson, M.L. Shuler. 2010. Macromolecular crowding can account for RNase-sensitive constraint of bacterial nucleoid structure. Biochem. Biophys. Res. Comm.. 395: 42-47.

• M.S. Ravenscroft-Chang, J. Stohlman, P. Molnar, A. Natarajan, H.E. Canavan, M. Teliska, M. Stancescu, V. Krauthamer and J.J. Hickman, “Altered Calcium Dynamics in Cardiac Cells Grown on Silane-Modified Surfaces,” Biomaterials 31:602-607 (2010).

• C.M. Smith, T.D. Roy, A. Bhalkikar, B. Li, J.J. Hickman and K.H. Church, “Engineering a Titanium and Polycaprolactone Construct for a Biocompatible Interface Between the Body and Artificial Limb,” Tissue Engineering Part A 16(2):717-724 (2010). X.F. Guo, K. Johe, P. Molnar, H. Davis, J.J. Hickman, “Characterization of a Human Spinal Cord Stem Cell Line and Its Induction to Motoneurons,” Tissue Engineering and Regenerative Medicine 4:181-193 (2010).

• K. Varghese, P. Molnar, M. Das, N. Bhargava, S. Lambert, M.S. Kindy and J.J. Hickman, “A New Target for Amyloid Beta Toxicity Validated by Standard and High-throughput Electrophysiology,” PLoS One 5(1): e8643. doi:10.1371/journal.pone.0008643 (2010).

• M. Das, J.W. Rumsey, N. Bhargava, M. Stancescu and J.J. Hickman, “A Defined Long-Term In Vitro Tissue Engineered Model of Neuromuscular Junctions,” Biomaterials 31:4880-4888 (2010).

• K. Wilson, M. Das, K.J. Wahl, R.J. Colton and J.J. Hickman, “Measurement of Contractile Stress Generated by Cultured Muscle on Silicon Cantilevers,” PLoS One 5(6): e11042. doi: 10.1371/journal.pone.0011042 (2010).

• D. Edwards, M. Das, P. Molnar and J.J. Hickman, “Addition of Glutamate to Serum Free Culture Promotes Recovery of Electrical Activity in Adult Hippocampal Neurons In Vitro,” J Neuroscience Methods 190:155-163 (2010).

• L. Chi, J. Yu, H. Zhu, X. Li, S. Zhu, Z. Li, L.C. Pettigrew, D. Grass, J.J. Hickman, M.S. Kindy, “Dual Neuronal Response to Tumor Necrosis Factor-alpha Following Spinal Cord Injury,” Neural Regeneration Research 5(12):917-926 (2010).

• J.W. Rumsey, M. Das, A. Bhalkikar, M. Stancecsu and J.J. Hickman, “Tissue Engineering the Mechanosensory Circuit of the Stretch Reflex Arc: Sensory Neuron Innervation of Intrafusal Muscle Fibers,” Biomaterials 31:8218-8227 (2010).

• N. Bhargava, M. Das, D. Edwards, M. Stancescu, J.-F. Kang and J.J. Hickman, “Coexpression of Glutamate Vesicular Transporter (VGLUT1) and Choline Acetyltransferase (ChAT) Proteins in Embryonic Rat Hippocampal Neurons in Culture,” In Vitro Cell Dev Animal 46:685-692 (2010).

• C. Wang, L. Ma, M. Hossain, H. Wang, S. Zou, J.J. Hickman, M. Su, “Direct visualization of molecular scale chemical adsorptions on solids using plasmonic nanoparticle arrays,” Sensors and Actuators, B:Chemical 150(2):667-672 (2010).

• C. Wang, M. Hossain, L. Ma, Z. Ma, J.J. Hickman, M. Su, “Highly Sensitive Thermal Detection of Thrombin Using Aptamer-Functionalized Phase Change Nanoparticles,” Biosensors & Bioelectronics 26(2):437-43 (2010).

• J.C. Henderson, M. Yacopucci, C.J. Chun, K. Lenghaus and J.J. Hickman, “Investigation of the Behavior of Serum Plasma in a Microfluidics System,” JVST B 28(5):1066-1069 (2010).

• X. Guo, M. Das, J. Rumsey, M. Gonzalez, M. Stancescu and J.J. Hickman, “Neuromuscular Junction Formation between Human Stem Cell derived Motoneurons and Rat Skeletal Muscle in a Defined System,” Tissue Engineering, Part C 16(6):1347-1355 (2010).

• M. Ramalingam, S.S. Liao, S. Ramakrishna, P. Molnar, Z.M. Huang, M. Kotaki, K. Panduranga Rao and J.J. Hickman, “Skeletal Regeneration Nanobiomaterials,” in Biomaterials for Bone, Regenerative Medicine, TTP Publishers, Swiss/USA, (2010).

• Echtenkamp, P.L., D.B. Wilson, and M.L. Shuler. 2009. Cell cycle progression in Escherichia coli b/r Affects transcription of certain genes: Implications for synthetic genome design. Biotechnol. Bioeng. 102(3):902-909.

• El Gheriany, I.A., D. Bocioaga, A.G. Hay, W.C. Ghiorse, M.L. Shuler, and L.W. Lion. 2009. Iron requirement for Mn(II) xxidation by Leptothrix discophora SS-1. Appl. Environ. Microbiol. 75(5):1229-1235.

• Shuler, M.L. and M.B. Esch. 2009. Micro cell culture analogs and what they can contribute to the drug screening process. www.alttox.org

• Kim, B.J., J.H. Park, T.H. Park, P.A. Bronstein, D.J. Schneider, S.W. Cartinhour, and M.L. Shuler. 2009. Effect of iron concentration on the growth rate of Pseudomonas syringae and the expression of virulence factors in hrp-inducing minimal medium. Appl. Environ. Microbiol. 75(9):2720-2726.

• Sung, J.H. and M.L. Shuler. 2009. Prevention of air bubble formation in a microfluidic perfusion cell culture system using a microscale bubble trap. Biomed. Microdevices 11: 731-738.

• Sung, J.H. and M.L. Shuler. 2009. A micro cell culture analog (microCCA) with 3-D hydrogel culture of multiple cell lines to assess metabolism-dependent cytotoxicity of anti-cancer drugs. Lab Chip 9:1385-1394.

• Mahler, G.J., M.L. Shuler, and R.P. Glahn. 2009. Characterization of Caco-2 and HT29-MTX co-cultures in an in vitro digestion/cell culture model used to predict iron bioavailability. J. Nutr. Biochem. 20:494-502.

• Sung, J.H., A. Dhiman, and M.L. Shuler. 2009. A combined pharmacokinetic-pharmacodynamic (PK-PD) model for tumor growth in the rat with UFT administration. J. Pharm. Sci. 98:1885-1904.

• Tatosian, D.A. and M.L. Shuler. 2009. A novel system for evaluation of drug mixtures for potential efficacy in treating multidrug resistant cancers. Biotechnol. Bioeng. 103:187-198.

• Xu, H. and M.L. Shuler. 2009. Quantification of chemical-polymer surface interactions in microfluidic cell culture devices. Biotechnol. Prog. 25:543-551.

• Mahler, G.J., M.B. Esch, R.P. Glahn, and M.L. Shuler. 2009. Characterization of a gastrointestinal tract microscale cell culture analog used to predict drug toxicity. Biotechnol. Bioeng. 104:193-205.

• Sung, J.H., J.R. Choi, D.H. Kim, M.L. Shuler. 2009. Fluorescence optical detection in situ for real-time monitoring of enzymatic activity of liver cells in multiple microfluidic devices. Biotechnol. Bioeng.104:516-525.

• Shin A.K., S.J. Kim, S.H. Lee, T.H. Park, K.M. Byun, S.G. Kim, M.L. Shuler. 2009. Detection of avian influenza-DNA hybridization using wavelength-scanning surface plasmon resonance biosensor. J Optical Society of Korea 13, (3): pp. 392-397.

• K. Varghese, M. Das, N. Bhargava, P. Molnar, M.S. Kind and J.J. Hickman, “Regeneration and Characterization of Adult Mouse Hippocampal Neurons in a Defined In Vitro System,” Journal of neuroscience methods 177:51-59 (2009).

• M. Ramalingam, P. Molnar, K. P. Rao and J. J. Hickman, “Biomaterial Surface Patterning Utilizing Self Assembled Monolayers to Control Neuronal Cell Behavior,” Int. Journal of Biomedical Engineering and Technology, Review 2(2):104-134 (2009).

• J.W. Rumsey, M. Das, M. Stancescu, M. Bott, C. Fernandez-Valle and J.J. Hickman, “Node of Ranvier Formation on Motoneurons In Vitro,” Biomaterials30:3567-3572 (2009).

• T. Xu, P, Molnar, C. Gregory, M. Das, T. Boland and J.J. Hickman, “Electrophysiological Characterization of Embryonic Hippocampal Neurons Cultured in 3D Collagen Hydrogel,” Biomaterials 30:4377-4383 (2009).

• M. Das, J. W. Rumsey, N. Bhargava, M. Stancescu and J.J. Hickman, “Skeletal Muscle Tissue Engineering: An Improved Model Promoting Long Term Survival of Myotubes, Structural Development of E-C Coupling Apparatus and Neonatal Myosin Heavy Chain (MHC) Expression,” Biomaterials 30:5392-5402 (2009).

• M. Das, J.W. Rumsey, N. Bhargava, C. Gregory, L. Riedel, J.F. Kang, J.J. Hickman, “Developing a Novel Serum-Free Cell Culture Model of Skeletal Muscle Differentiation by Systematically Studying the Role of Different Growth Factors in Myotube Formation,” In Vitro Cell Dev Biol Animal 45:378-387 (2009).

• N. Akanda, M. Stancescu, P. Molnar and J.J. Hickman, “Analysis of Toxin Induced Changes in Action Potential Shape for Drug Development,” Journal of Biomolecular Screening 14(10):1228-1235 (2009).

• N. Bhargava, M. Das, A. S. Karakoti, S. Patil, J.F. Kang, M. Stancescu, M. S. Kindy, S. Seal and J.J. Hickman, “Regeneration of Adult Mice Motoneurons Utilizing a Defined System and Anti-oxidant Nanoparticles,” Journal of Nanoneuroscience 1(2):130-143 (2009).

• D.G. Taylor, A. Natarajan, B. Moscato, P. Molnar, J.J. Hickman, S.N. Ebert, “Adrenergic Deficiency Leads to Slowed Ventricular Conduction and Increased Arrhythmias in Embryonic Mouse Hearts,” Circulation 120(18):S616 (2009).

• M. Das, N. Bhargava, A. Bhalkikar, J. F. Kang and J. J. Hickman, “Temporal Neurotransmitter Conditioning Restores the Functional Activity of Adult Spinal-Cord Neurons in Long-Term Culture,” Experimental Neurology 209:171-180 (2008).

• J.W. Rumsey, M. Das, J.F. Kang, R. Wagner, P. Molnar and J.J Hickman, “Tissue Engineering Intrafusal Fibers: Dose and Time Dependent Differentiation of Nuclear Bag Fibers in a Defined In Vitro System using Neuregulin 1-β-1,” Biomaterials 29:994-1104 (2008).

• J. Liu, J.W. Rumsey, P. Molnar, M. Das, C. Gregory, L. Riedel and J.J. Hickman, “Electrophysiological and Immunocytochemical Characterization of DRG Neurons on an Organosilane Surface in Serum Free Medium,” In Vitro Cellular & Developmental Biology 44:162-168 (2008).

• A. Natarajan, C.J. Chun, J.J. Hickman and P. Molnar, "Growth and Electrophysiological Properties of Rat Embryonic Cardiomyocytes on Hydroxyl- and Carboxyl-Modified Surfaces,” Journal of Biomaterials Science: Polymer Edition 19(10):1319-1331 (2008).

• V. Thakore, A. Behal, P. Molnar, D.C. Leistritz, and J.J. Hickman, “Nanoscale Nonlinear Dynamic Characterization of the Neuron-Electrode Junction,” J. Comput. Theor. Nanosci. 5(11):2164-2169 (2008).

• McAuliffe, G.J., J.Y. Chang, R.P. Glahn, and M.L. Shuler.  "Development of a gastrointestinal tract microscale cell culture analog to predict drug transport."  Mol. Cell Biomech. 5(2):119-132 (2008).

• J. Rolland, K-S. Lee, A. Mahmood, L. Fluck, J. Duarte, I. Kaya, A. Santhanam, P. Meemon, S. Murali, O. Ilegbusi, P. Kupelian, A.M. Kachurin, W.L. Warren, P. Molnar, J.J. Hickman and P.K. Kolattukudy, Eds J.D. Westwood, R.S. Haluck, H.M.Hoffman, G.T. Mogel, R. Phillips, R.A. Robb and K.G. Vosburgh, “Collaborative Engineering: 3-D Optical Imaging and Gas Exchange Simulation of In-Vitro Alveolar Constructs,” in Studies in Health Technolology Informatics 132:426-432 (2008).

• Rolland, J. P., K. S. Lee, A. Mahmood, L. Fluck, J. Duarte, I. Kaya, A. Santhanam, P. Meemon, S. Murali, O. Ilegbusi, P. Kupelian, W. L. Warren, P. Molnar, J. J. Hickman and P. E. Kolattukudy. Collaborative Engineering: 3-D Optical Imaging and Gas Exchange Simulation of In-Vitro Alveolar Constructs. Medicine Meets Virtual Reality 16 - parallel, combinatorial, convergent: NextMed by Design. J. D. Westwood, R. S. Haluck, H. M. Hoffmanet al. 132. (2008).

• M. Ramalingam, P, Molnar and J.J. Hickman, “Nano-Surface Patterning Techniques for Cell Studies,” Encyclopedia of Nanoscience and Nanotechnology(ENN) American Scientific Publishers, Valencia, California, (2008).

• M. Ramalingam, S. Ramakrishna and J.J. Hickman, “Cell Responsive Nano-fibrous Materials for Tissue Engineering,” in Encyclopedia of Nanoscience and Nanotechnology, American Scientific Publishers, Ed. Hari Singh Nalwa, Valencia, California, (2008).

• M. Das, S. Patil, N. Bhargava, J.F. Kang, L. Riedel, S. Seal and J.J. Hickman, “Auto-catalytic Ceria Nanoparticles Offer Neuroprotection to Adult Rat Spinal Cord Neurons,” Biomaterials 28:1918-1925 (2007).

• P. Molnar, W. Wang, A. Natarajan, J.W. Rumsey and J.J. Hickman, “Photolithographic Patterning of C2C12 Myotubes using Vitronectin as Growth Substrate in Serum-Free Medium,” Biotechnology Progress 23(1): 265-268 (2007).

• M. Das, J.W. Rumsey, C.A. Gregory, N. Bhargava, J.F. Kang, P. Molnar, L. Riedel, J.J. Hickman, “Embryonic Motor Neuron-Skeletal Muscle Co-culture in a Defined System,” Neuroscience 146:481-488 (2007).

• Cheng, S-Y., S. Heilman, M. Wasserman, S. Archer, M.L. Shuler, and M. Wu.  "A hydrogel-based microfluidic device for the studies of directed cell migration."  Lab Chip 7:763-769 (2007).

• K. Wilson, P. Molnar and J.J. Hickman, “Integration of Functional Myotubes with a Bio-MEMS Device for Non-Invasive Interrogation,” Lab-on-a-Chip 7:920-922 (2007).

• Oh, Taek-il, J.H. Sung, D.A. Tatosian, M.L. Shuler, and D. Kim.  "Real-time fluorescence detection of multiple microscale cell culture analog devices in Situ."  Cytometry part A 71A:857-865 (2007).

• M. Das, K. Wilson, P. Molnar and J.J. Hickman, “Differentiation of Skeletal Muscle and Integration of Myotubes with Silicon Microstructures using Serum-Free Medium and a Synthetic Silane Substrate,” Nature Protocols 2(7):1795-1801 (2007).

• P. Molnar, J.J. Hickman, “Modeling of action potential generation in NG108-15 cells,” Methods Mol. Biol. 403:175-84 (2007).

• P. Molnar P, JF. Kang, N. Bhargava, M. Das and J.J. Hickman, “Synaptic connectivity in engineered neuronal networks.” Methods Mol. Biol. 403:165-73 (2007).

• P. Molnar, J.F. Kang, N. Bhargava, M. Das and J.J. Hickman, “Synaptic Connectivity in Engineered Neuronal Networks,” in Patch Clamp Methods and Protocols Humana Press Methods in Molecular Biology Series, (July 2007).

• P. Molnar and J.J. Hickman, “Modeling of Action Potential Generation inNG108-15 Cells,” in Patch Clamp Methods and Protocols Humana Press Methods in Molecular Biology Series, (July 2007).

• A. Natarajan, P. Molnar, K. Sieverdes, K. Jamshidi and J.J. Hickman, “Multielectrode Recordings of Cardiac Action Potentials as a High Throughput Method to Evaluate Pesticide Toxicity,” Toxicology In Vitro 20(3):375-381 (2006).

• D. K Mohan, P. Molnar and J.J. Hickman. “Toxin detection based on action potential shape analysis using a realistic mathematical model of differentiated NG108-15 cells,” Biosensors & Bioelectronics 21:1804-1811 (2006).

• M. Das, C. Gregory, K. Wilson, P. Molnar, L. Riedel and J.J. Hickman, “A Defined System to Allow Skeletal Muscle Differentiation and Subsequent Integration with Silicon Microstructures,” Biomaterials 27(24):4374-4380 (2006).

• S. Krishnamoorthy, J. Feng, A. Henry, L. Locascio, J.J. Hickman and S. Sundaram, “Simulation and Experimental Characterization of Electroosmotic Flow in Surface Modified Channels,” Microfluidics and Nanofluidics 2:345-355 (2006).

• D. Henry, K. Lenghaus, K.A. Wilson, M. Hirsch-Kuchma, J. Jenkins, S. Sundaram and J.J. Hickman, “Submonolayer Measurements of Adsorbed Proteins In Microfluidic Channels,” in Bionanotechnology: Proteins to Nanodevices (V. Renugopalakrishnan, Randy V Lewis, Pawan K Dhar), Dordrecht, The Netherlands: Springer Press, (2006).

• Ma, S.H., L.A. Lepak, R.J. Hussain, W. Shain, and M.L. Shuler.  "An endothelial and astrocyte co-culture model of the blood-brain barrier utilizing an ultra-thin, nanofabricated silicon nitride membrane."  Lab Chip 5:74-85 (2005).

• T. Xu, J. Jin, C. Gregory, J.J. Hickman and T. Boland, “Inkjet Printing of Viable Mammalian Cells,” Biomaterials 26(1):93-99 (2005).

• A. Behal, D.M. Dawson, P. Molnar and J.J. Hickman, “The stretch reflex arc: simulation, control and identification” (2005)

• M. Das, N. Bhargava, C. Gregory, L. Riedel, P. Molnar and J.J. Hickman, “Adult Rat Spinal Cord Culture on an Organosilane Surface in a Novel Serum-Free Medium” In Vitro Cell Dev Biol- Animal 41:343-348 (2005).

• J.J. Hickman, “Building Minimalistic Hybrid Neuroelectric Devices,” in Toward Replacement Parts for the Brain: Implantable Biomimetic Electronics as Neural Prosthetic (T.W. Berger and D.L. Glanzman Eds.), 1st edition. Cambridge, MA: MIT Press (2005).

• J.J. Hickman, P. Molnar, M. Hirsch-Kuchma, J.W. Rumsey, K. Wilson “Biosurface Engineering, Cell Patterning,” in Wiley Encyclopedia of Medical Devices and Instrumentation (John G. Webster), 2nd edition. Hoboken, NJ: John Wiley & Sons, Inc. (2005).

• T. Xu, S. Petridou, E. Lee, E. Roth, N. Vyavahare, J.J. Hickman and T. Boland, “Construction of High-Density Bacterial Colony Arrays and Patterns by the Ink-jet Method,” Journal of Biotechnology & Bioengineering 85:29 (2004).

• Sin, A., K.C. Chin, M.F. Jamil, Y. Kostov, G. Rao, and M.L. Shuler.  "The design and fabrication of three-chamber microscale cell culture analog devices with integrated dissolved oxygen sensors."  Biotechnol. Prog. 20:338-345 (2004).

• E. A. Roth, T. Xu, M. Das, C. Gregory, J.J. Hickman and T. Boland, “Inkjet Printing for High Throughput Cell Patterning,” Biomaterials 25(17): 3707-3715 (2004).

• Viravaidya, K., A. Sin, and M.L. Shuler.  "Development of a microscale cell culture analog to probe naphthalene toxicity."  Biotechnol. Prog. 20:316-323 (2004).

• J.K. Evju, P.B. Howell, L.E. Locascio, M.J. Tarlov and J.J. Hickman, “Atmospheric Pressure Microplasmas for Modifying Sealed Microfluidic Devices,” Applied Physics Letters 84(10):1668-1670 (2004).

• Viravaidya, K. and M.L. Shuler.  "Incorporation of 3T3-L1 cells to mimic bioaccumulation in a microscale cell culture analog device for toxicity studies."  Biotechnol. Prog. 20:590-597 (2004). 

• B. Sweryda-Krawiec, H. Devaraj, G. Jacob, and J.J. Hickman, “A New Interpretation of Serum Albumin Surface Passivation,” Langmuir 20(6):2054-2056 (2004).

• Sin, A., C.F. Reardon, and M.L. Shuler.  "A self-priming microfluidic diaphragm pump capable of recirculation fabricated by combined soft lithography and traditional machining."  Biotechnol. Bioeng. 85(3):359-363 (2004).

• M. Das, P Molnar, C. Gregory, L. Riedel and J.J. Hickman, “Long-term Culture Of Embyonic Rat Cardiomyocytes on an Organosilane Surface in a Serum Free Medium,” Biomaterials 25(25):5643-5647 (2004).

• Viravaidya, K., E. Jan, and M.L. Shuler.  "Lipid-gel and PDMS film to mimic bioaccumulation in the adipocytes."  Biotechnol. Bioeng. 86(6):643-649 (2004).

• F. Jin, J.J. Hickman, K. Lenghaus and R. Marcus, “Total Protein Determinations by Particle Beam/Hollow Cathode Optical Emission Spectroscopy (PB/HC-OES)System II: Improved Sensitivity Through Use of Silica Micro-particles as Carrier Agents for Enhanced Particle Transport,” Journal of Analytical Atomic Spectrometry 19(9):1199-1205 (2004).

• F. Jin, J.J. Hickman, K. Lenghaus, R. Marcus, “Total Protein Determinations by Particle Beam/Hollow Cathode Optical Emission Spectroscopy (PB/HC-OES) System III: Investigation of Carrier Salts for Enhanced Particle Transport,” Anal Bioanal Chem. 380 (2):204-211 (2004).

• J. Jenkins, B. Prabhakarpandian., K. Lenghaus, J.J. Hickman, S. Sundaram, “Fluidics Resolved Analysis of Protein Adsorption in a Biomicrofluidic System,” Analytical Biochem 331:207-215 (2004).

• J.J. Hickman, C. Zou, D. Ofer, P.D. Harvey, M.S. Wrighton, P.E. Laibinis, C.D. Bain and G.M. Whitesides, “Combining Spontaneous Molecular Assembly with Microfabrication to Pattern Surfaces: Selective Binding of Isonitriles to Platinum Microwires and Characterization by Electrochemistry and Surface Spectroscopy,” J. Am. Chem. Soc. 111:7271 (1989).

• P.E. Laibinis, J.J. Hickman, M.S. Wrighton and G.M. Whitesides, “Orthogonal Systems for Self-Assembled Monolayers: Alkanethiols on Gold and Alkane Carboxylic Acids on Alumina,” Science 245:845 (1989).

• J.J. Hickman, D. Ofer, C. Zou, M.S. Wrighton, P.E. Laibinis and G.M. Whitesides, “Selective Functionalization of Gold Microstructures with Ferrocenyl Derivatives via Reaction With Thiols or Disulfides: Characterization by Electrochemistry and Auger Electron Spectroscopy,” J. Am. Chem. Soc. 113:1128 (1991).

• J.J. Hickman, D. Ofer, P.E. Laibinis, G.M. Whitesides and M.S. Wrighton, “Molecular Self-Assembly of Two-Terminal, Voltammetric Microsensors With Internal References,” Science 252:688 (1991).

• J.J. Hickman and M.S. Wrighton, “Face-Specific Interactions of Anionic Sulfur Donors with Oriented Crystals of (0001) CdX (X = Se, S) and Correlation with Electrochemical Properties,” J. Am. Chem. Soc. 113:4440 (1991).

• J.J. Hickman, P.E. Laibinis, D.I. Auerbach, C. Zou, T.J. Gardner, G.M. Whitesides and M.S. Wrighton, “Toward Orthogonal Self-Assembly of Redox Active Molecules on Pt and Au: Selective Reaction of Disulfide with Au and Isonitrile with Pt,” Langmuir 8:357 (1992).

• S.K. Bhatia, J.J. Hickman and F.S. Ligler, “A New Approach to Engineering Patterned Biomolecular Assemblies,” J. Am. Chem. Soc. 114:4432 (1992).

• D.A. Stenger, J.H. Georger, C.S. Dulcey, J.J. Hickman, A.S. Rudolph, T.B. Nielson, S. McCort, and J.M. Calvert, “Coplanar Molecular Assemblies of Amino- and Perfluorinated Alkylsilanes: Characterization and Geometric Definition of Mammalian Cell Cultures,” J. Am. Chem. Soc. 114:8435 (1992).

• J.J. Hickman, G.L. Bergeron, M.A. Czarnaski, and D.A. Kirkpatrick, “Surface Chemistry of TaSi2-Si Eutectic Cold Cathodes and the Effect on Vacuum Field Emission,” Appl. Phys. Lett. 61:2518 (1992).

• D.A. Stenger, C. Pike, J.J. Hickman and C.W. Cotman, “Surface Determinants of Neuronal Survival and Growth on Self-Assembled Monolayers in Culture," Brain Res. 630:136 (1993).

• B.J. Spargo, M.A. Testoff, T.B. Nielsen, D.A. Stenger, J.J. Hickman and A.S. Rudolph, “Adhesion, Spreading, and Differentiation of Endothelial Cells on Self-Assembled Amino- and Perfluoro-Alkylsilane Monolayers,” Proc. Nat. Acad. Sci. USA 91:11070 (1994).

• J.J. Hickman and D.A. Stenger, “Interactions of Cultured Neurons with Defined Surfaces,” in: Enabling Technologies for Cultured Neural Networks (D.A. Stenger and T.M. McKenna, Eds.), 1st edition. San Diego: Academic Press, Inc. 51 (1994).

• D.A. Stenger and J.J. Hickman, “Lithographic Definition of Neuronal Microcircuits,” in: Enabling Technologies for Cultured Neural Networks (D.A. Stenger and T.M. McKenna, Eds.), 1st edition. San Diego: Academic Press, Inc. 77 (1994).

• Sweeney, L.M., M.L. Shuler, J.G. Babish, and A. Ghanem.  "A cell culture analog of rodent physiology:  Application to naphthalene toxicology." in: Toxicol. in Vitro 9(3):307-316 (1995).

• A.E. Schaffner, J.L. Barker, D.A. Stenger and J.J. Hickman, “Investigation of the Factors Necessary for Growth of Hippocampal Neurons in a Defined System,” J. Neuroscience Methods 62:111 (1995).

• Mufti, N.A., N.A. Bleckwenn, J.G. Babish, and M.L. Shuler.  "Possible involvement of the Ah receptor in the induction of cytochrome P-450IA1 under conditions of hydrodynamic shear in microcarrier-attached hematoma cell lines." Biochem. Biophys. Res. Commun. 208(1):144-15 (1995). 

• Sweeney, L.M., M.L. Shuler, D.J. Quick, and J.G. Babish.  "A preliminary physiologically-based pharmacokinetic model for naphthalene and naphthalene oxide in mice and rats."  Ann. Biomedical Eng. 24:305-320 (1996).
• Shuler, M.L., A. Ghanem, D. Quick, M.C. Wong, and P. Miller.  "A self-regulating cell culture analog device to mimic animal and human toxicological responses."  Biotechnol. Bioeng. 52:45-60 (1996).

• D.R. Jung, D.S. Cuttino, J.J. Pancrazio, P. Manos, T. Custer, R.S. Sathanoori, L.E. Aloi, M.G. Coulombe, M.A. Czarnaski, D.A. Borkholder, G.T. Kovacs, P. Bey, D.A. Stenger and J.J. Hickman, “Cell-Based Sensor Microelectrode Array Characterized by Imaging XPS, SEM, Impedance Spectroscopy, and Cellular Recordings,” J. Vac. Sci. Tech. A 16:1183 (1998).

• D.A. Stenger, J.J. Hickman, K.E. Bateman, M.S. Ravenscroft, W. Ma, J.J. Pancrazio, K.M. Shaffer, A.E. Schaffner, D.H. Cribbs and C.W. Cotman, “Microlithographic Definition of Axonal/Dendritic Polarity in Cultured Hippocampal Neurons,” J. Neuroscience Methods 82:167 (1998).

• M.S. Ravenscroft, K.E. Bateman, K.M. Shaffer, H.M. Schessler, D.R. Jung, T.W. Schneider, C.B. Montgomery, T.L. Custer, A.E. Schaffner, Q.Y. Liu, Y.X. Li, J.L. Barker and J.J. Hickman, “Developmental Neurobiology Implications from Fabrication and Analysis of Hippocampal Neuronal Networks on Patterned Silane-Modified Surfaces,” J. Amer. Chem. Soc. 120:12169 (1998).

• Mufti, N.A. and M.L. Shuler.  "Different In vitro systems affect CYPIA1 activity in response to 2,3,7,8-Tetrachlorodibenzo-p-dioxin."  Toxicol. in Vitro 12:259-272 (1998).
• Quick, D.J. and M.L. Shuler.  "Use of in vitro data for construction of a physiologically based pharmaceokinetic model for naphthalene in rats and mice to probe species differences."  Biotechnol. Prog. 15:540-555 (1999).
• Ghanem, A. and M.L. Shuler.  2000.  Combining cell culture analogue reactor designs and PBPK models to probe mechanisms of naphthalene toxicity.  Biotechnol. Prog. 16(3):33-345. 
  
• Sin A, G.T. Baxter, M.L. Shuler, "Animal on a chip: a microscale cell culture analog device for evaluting toxicological and pharmacological profiles." Proc. SPIE 4560, Microfluidics and BioMEMS, 98-101 (2001).
  
• Park, T-H. and M.L. Shuler. "Integration of cell culture and microfabrication technology."  Biotechnol. Prog. 19:243-253 (2003).

• K. Lenghaus, J. Dale, C. Henderson, D. Henry, E. Loghin and J.J. Hickman, “Enzymes as Ultra-Sensitive Probes for Adsorption in Flow Systems,” Langmuir19:5971 (2003).